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Sensitive, selective, and rapid detection of a single biomarker in complex biological samples is the ultimate goal of different bioanalytical techniques. Immunosensor is an analytical device consisting of antibodies or antigens immobilized on the surface of the signal transducer. Upon biomarker binding and immune complex formation the physicochemical changes on the surface are converted to a measureable signal proportional to the biomarker concentration. Various biomarker detection methods and different analytical signal amplification strategies offer new possibilities to improve the performance of immunosensors. Additionally, characteristics of an immunosensor such as sensitivity, stability, regeneration ability depend on the type and amount of immobilized antibodies, on the remaining activity of antigen binding sites after immobilization and on the orientation on the sensing surface (Fig. 1) [1,2]. Proper immobilization of antibodies is of considerable interest in many fields of interest, e.g. in clinical diagnostics (for the specific cancer biomarkers, different hormones detection), in food safety analysis or environmental monitoring. Different nanomaterials and nanostructured surfaces have a considerable impact on the analytical characteristics and performance of immunosensors [3-7].